The EpiQuik™ Plant ChIP Kit is suitable for combining the specificity of immunoprecipitation with qualitative and quantitative PCR, MS-PCR, DNA sequencing, and southern blot, as well as DNA microarray.
Like using other ChIP kits, if you use the EpiQuik™ Plant ChIP Kit, choice of a good antibody (i.e. IP proved) is required for precipitating the fixed protein/DNA complexes.
The kit size is designed for 24 or 48 ChIP reactions, not for 24 or 48 samples. The standard protocol of the kit allows for performing 8 reactions with one sample. For testing more samples, the amount of each sample should be reduced. The amount of each component of the kit or reagents used for chromatin preparation should be also proportionally reduced.
艾美捷植物染色质免疫沉淀(ChIP)试剂盒#P-2014-24可以有效进行体外检测植物中蛋白质-DNA相互作用,操作方便.操作过程不到六小时就可以获得比其它试剂盒更好的结果.试剂盒获得的染色质可用来进行定性和定量PCR、southern 印迹以及DNA微阵列分析.
植物染色质免疫沉淀(ChIP)试剂盒提供了对植物细胞样品进行染色质免疫沉淀反应所需的所有试剂.并且,试剂盒中还有一种ChIP级二甲基组蛋白H3-K9抗体和一种阴性对照抗体(正常小鼠的IgG).从细胞样品中提取出来的染色质进行适当的打断,然后加入到微孔中与其表面上吸附的抗体发生免疫反应.特异性结合到微孔上的DNA从抗体-捕获蛋白-DNA复合物上释放出来,翻转后,通过本公司专门设计的高速离心柱纯化.洗脱下来的DNA可用于随后的各种分析.
该植物染色质免疫沉淀(ChIP)试剂盒运输和储存:
该试剂盒分为两部分运输:一部分在室温下运输,另一部分在4°C下的冷冻冰袋。
收到后:(1)将以下成分储存在4°C下:蛋白酶抑制剂鸡尾酒、非免疫IgG、蛋白酶K、抗二甲基H3-K9和8孔检测条。(2) 存储所有其他室温下的组件。
该试剂盒在正确储存的情况下,自发货之日起可稳定长达6个月。
该植物染色质免疫沉淀(ChIP)试剂盒特点:
1、市场上同类产品中较为快捷的试剂盒,整个处理过程不到6小时;
2、96孔板模式使研究人员能根据自己需要选择手工或是高通量分析;
3、附有DNA纯化柱子:大量减少不必要的重复劳动;
4、操作简便、结果可靠、统一的分析条件;
部分引文参考:
Zhang L et. al. (August 2020). The HY5 and MYB15 transcription factors positively regulate cold tolerance in tomato via the CBF pathway. Plant Cell Environ.
Li Y et. al. (July 2020). MYB repressors and MBW activation complex collaborate to fine-tune flower coloration in Freesia hybrida. Commun Biol. 3(1):396.
He et. al. (June 2020). The phytochrome-interacting transcription factor CsPIF8 contributes to cold tolerance in citrus by regulating superoxide dismutase expression Plant Science. 298